UJI FITOKIMIA DAN EFEKTIVITAS ANALGESIK DAUN MUDA DAN DAUN TUA MATOA (Pometia pinnata J.R.Forst. & G.Forst.) PADA MENCIT JANTAN (Mus musculus) YANG DIINDUKSI ASAM ASETAT 1% Ni Wayan Hely Adnyani Dewi Fakultas Kesehatan Program Studi Sarjana Farmasi Klinik Dan Komunitas Institut Teknologi dan Kesehatan Bali Email: [email protected] ABSTRAK Latar Belakang: Analgesik adalah senyawa yang dapat mengurangi rasa nyeri. Daun matoa (Pometia pinnata J.R.Forst. & G.Forst.) diketahui memiliki efek sebagai analgesik dengan kandungan metabolit sekunder yaitu flavonoid. Tujuan: Penelitian ini bertujuan mengetahui kandungan metabolit sekunder dan efektivitas analgesik dari daun muda dan daun tua matoa pada mencit jantan (Mus musculus) yang diinduksi asam asetat 1%. Metode: Metode penelitian ini menggunakan desain penelitian true experimental study dengan rancangan post-test only control grup design dan menggunakan 28 ekor mencit jantan (Mus Musculus). Data yang diperoleh dianalisis dengan Shapiro wilk, dilanjutkan dengan uji one way ANOVA dan uji post hoc LSD. Hasil: Hasil penelitian menunjukkan bahwa ekstrak etanol daun muda dan daun tua matoa (50mg/KgBB) mengandung senyawa saponin, tannin, alkaloid, fenolik, dan steroid, sementara flavonoid hanya terkadung pada daun tua. Hasil analisis statistik menunjukkan bahwa aktivitas daun muda dan daun tua matoa memiliki perbedaan bermakna dalam menurunkan nyeri. Daun tua memiliki daya penurunan nyeri lebih baik dari pada daun muda matoa dengan nilai proteksi >50% yaitu 53,80%. Kesimpulan: Ekstrak etanol daun muda dan daun tua matoa (Pometia pinnata J.R.Forst & G.Forst) dosis 50mg/KgBB memiliki efektivitas sebagai analgesik terhadap mencit jantan yang diinduksi asam asetat 1%. Kata Kunci: Analgesik, Matoa (Pometia pinnata J.R.Forst & G.Forst), Writhing test, Asam asetat

THE PHYTOCHEMICAL TEST AND THE EFFECTIVENESS OF ANALGESIC FROM THE LEAVES OF YOUNG AND OLD MATOA (Pometia pinnata J.R.Forst. & G.Forst.) IN MALE MICE (Mus musculus) INDUCED BY 1% ACETIC ACID Ni Wayan Hely Adnyani Dewi Faculty of Health Bachelor of Clinical and Community Pharmacy Institute of Technology and Health Bali Email: [email protected] ABSTRACT Background: Analgesics are compounds that can reduce pain. Matoa leaves (Pometia pinnata J.R.Forst. & G.Forst.) have an analgesic effect with the content of secondary metabolites, namely flavonoids. Purpose: The purpose of this study was to determine the content of secondary metabolites and the effectiveness of analgesics from the leaves of young and old matoa in male mice (Mus musculus) induced by 1% acetic acid. Method: This study employed a true experimental design with a post-test-only control group design and used 28 male mice (Mus Musculus). The data obtained were analyzed with Shapiro Wilk, followed by one way ANOVA test and LSD post hoc test. Results: Findings indicated that the ethanol extracts of the young and old leaves of matoa (50 mg/KgBB) contained saponins, tannins, alkaloids, phenolics, and steroids, while flavonoids were only in old leaves. The statistical analysis results showed that the activity of young leaves and old leaves of matoa significantly differed significantly in reducing pain. Old leaves had better pain reduction ability than the young matoa leaves, with a protection value of >50%, precisely at 53.80%. Conclusion: Ethanol extracts of young and old leaves of matoa (Pometia pinnata J.R.Forst & G.Forst) dose of 50mg/KgBW effective as analgesics in male mice induced by 1% acetic acid. Keywords: Analgesics, Matoa (Pometia pinnata J.R.Forst & G.Forst), Writhing test, Acetic acid