Uji Fitokimia dan Perbandingan Uji Toksisitas Ekstrak Etanol Daun Muda dan Daun Tua Kopi Arabika (Coffea arabica L.) Kintamani Bali Dengan Metode Brine Shrimp Lethality Test (BSLT) Putu Tri Ari Suari Fakultas Kesehatan Program Studi Sarjana Farmasi Klinik dan Komunitas Institut Teknologi Dan Kesehatan Bali E-mail: [email protected] ABSTRAK Daun kopi arabika diketahui memiliki aktivitas farmakologi seperti antihiperkolesterolemia, hepatoprotektor, anti inflamasi, memiliki aktivitas antioksidan dan menurunkan kadar glukosa darah. Penelitian sebelumnya telah menunjukkan bahwa daun kopi arabika memiliki potensi aktivitas farmakologis namun belum dilakukan uji toksisitas akut dengan metode Brine Shrimp Lethality Test (BSLT) menggunakan larva udang artemia sebagai hewan uji. Penelitian ini bertujuan untuk mengetahui kandungan fitokimia serta perbandingan toksisitas pada daun muda dan daun tua kopi arabika (Coffea arabica L) Kintamani Bali Metode true experiment dengan rancangan studi post-test control group digunakan dalam penelitian ini. Metode sampling yang digunakan adalah simple random sampling. Data yang diperoleh dianalisis dengan probit. Kromatografi Lapis Tipis digunakan dalam penentuan kandungan fitokimia. Hasil penelitian menunjukkan adanya flavonoid, alkaloid, saponin, tannin, dan steroid pada ekstrak etanol daun muda dan daun tua kopi arabika (Coffea arabica L) Kintamani Bali. Nilai LC50 yang diperoleh dari ekstrak daun muda dan daun tua kopi arabika (Coffea arabica L) Kintamani Bali (0 ppm, 20 ppm, 50 ppm, 100 ppm, 250 ppm, 500 ppm, 1000 ppm) secara berturut-turut adalah 218,398 μg/mL dan 402,198 μg/mL. Ekstrak etanol daun muda dan daun tua arabika (Coffea arabica L) Kintamani Bali memiliki kandungan fitokimia yang sama. Ekstrak etanol daun muda lebih toksik daripada ekstrak etanol daun tua Kata kunci: Uji Fitokimia, BSLT, LC50,Toksisitas, Daun Kopi Arabika

The Phytochemical and Toxicity Test Between Ethanol Extract From Young And Old Leaves of Arabica Coffee (Coffea arabica L.) of Kintamani Bali Using Brine Shrimp Lethality Test (BSLT) Method Putu Tri Ari Suari Faculty of Health Bachelor of Clinical and Community Pharmacy Institute of Technology and Health Bali Email: [email protected] ABSTRACT Background. Arabica coffee leaves have pharmacological activities such as antihypercholesterolemia, hepatoprotective, anti-inflammation, antioxidant activity, and lowering blood glucose levels. Previous research has shown that arabica coffee leaves have potential pharmacological activity; however, an acute toxicity test has not been carried out using the Brine Shrimp Lethality Test (BSLT) method with artemia shrimp larvae as test subjects. The purpose of this study was to determine the phytochemical content and toxicity ratio in young and old leaves of Arabica coffee (Coffea arabica L.) of Kintamani Bali. Method. This study employed a true experiment method with a post-test control group study design. The sampling method used was simple random sampling. The data obtained were analyzed using probit. Results. The findings indicated that Thin Layer Chromatography (TLC) was used to determine phytochemical content. The substances contained were flavonoids, alkaloids, saponins, tannins, and steroids in the ethanol extract of young and old leaves of Arabica coffee (Coffea arabica L) Kintamani Bali. The value of LC50 obtained from young and old leaves of Arabica coffee (Coffea arabica L.) Kintamani Bali (0 ppm, 20 ppm, 50 ppm, 100 ppm, 250 ppm, 500 ppm, 1000 ppm), respectively, were 218.398 μg/mL and 402.198 μg/mL. Conclusion. The ethanol extracts of young and old leaves of Arabica coffee (Coffea arabica L.) of Kintamani Bali have the same phytochemical content. The ethanol extract of young leaves is more toxic than that of old leaves. Keywords: Phytochemical Test, BSLT, LC50, Toxicity, Kintamani Bali Arabica Coffee Leaves