Latar Belakang. Skrining pranikah sangat penting untuk kehamilan sehat, mencegah komplikasi, dan menurunkan angka kematian ibu dan bayi. Keberhasilannya bergantung pada pengetahuan dan kesiapan tenaga kesehatan. Tujuan penelitian ini mengetahui hubungan antara pengetahuan dan kesiapan tenaga kesehatan dengan capaian skrining pranikah di Puskesmas Kabupaten Klungkung. Metode. Penelitian ini menggunakan desain kuantitatif analitik korelasional dengan pendekatan cross-sectional. Sampel terdiri dari 167 bidan di enam Puskesmas Kabupaten Klungkung, yang dipilih secara total sampling. Data dikumpulkan melalui kuesioner dan dianalisis menggunakan uji Spearman Rho. Hasil. sebagian besar tenaga kesehatan berpengetahuan baik (81,07%) dan sangat siap (61,68%), pengetahuan tidak berhubungan signifikan dengan capaian skrining p=0,490). Namun, kesiapan tenaga kesehatan memiliki hubungan signifikan dengan capaian (p=0,033), meskipun korelasinya rendah (r=0,165) Kesimpulan. Kesiapan tenaga kesehatan berperan dalam capaian skrining pranikah. Pengetahuan saja tidak cukup perlu penguatan kesiapan dan dukungan eksternal lainnya. Kata Kunci : Skrining, Pengetahuan, Kesiapan, Capaian, Bidan.

Background. Premarital screening is important for healthy pregnancies, preventing complications, and decreasing maternal and infant mortality rates. Its success depends on the knowledge and readiness of health workers. Purpose: To determine the relationship between health workers’ knowledge and readiness on the achievement of premarital screening at Public Health Center in Klungkung Regency. Method. This research employed quantitative correlational analytical design with cross-sectional approach. The samples consisted of 167 midwives from six Public Health Center in Klungkung Regency which were chosen by using total sampling technique. The data were collected by using questionnaire and analyzed using the Spearman’s Rho test. Findings. The result showed that most of health worker had good knowledge (81.07%) and were highly ready (61.68%). There was no relationship between knowledge and screening achievement (p = 0.490). However, there was significant relationship between readiness and screening achievement (p = 0.033), although the correlation was weak (r = 0.165). Conclusion. Health workers’ readiness contributes to the achievement of premarital screening. Not only knowledge but also readiness and external support are important. Keywords: Screening, Knowledge, Readiness, Achievement, Midwife