Latar Belakang: Skrining pranikah adalah pemeriksaan kesehatan sebelum menikah guna mempersiapkan kehamilan sehat dan mencegah risiko penyakit genetik serta menular. Dukungan keluarga berperan penting dalam meningkatkan kesiapan wanita usia subur (WUS) menjalani skrining tersebut. Penelitian ini bertujuan mengetahui hubungan antara dukungan keluarga dan kesiapan WUS dalam melakukan skrining pranikah di UPTD Puskesmas II Sukawati. Metode: Penelitian menggunakan desain analitik korelasional dengan pendekatan cross-sectional. Jumlah respondenyaitu104 WUSyang dipilih dengan teknik consecutive sampling.Pengumpulan data melalui kuesioner dan analisis menggunakan uji Spearman Rho. Hasil: Hasil penelitian menunjukkan mayoritas responden memiliki dukungan keluarga tinggi dengan rata-rata nilai dukungan sebesar 41,88 dan nilai modus 51 dan kesiapan baikdengan nilai rata-rata 7,79. Uji statistik menunjukkan hubungan signifikan antara dukungan keluarga dan kesiapan WUS dalam skrining pranikah (p

Background: Premarital screening is a health examination which is conducted before marriage in order to prepare healthy pregnancy and prevent the risk of genetic and infectious diseases. Family support plays an important role to increase the readiness of women of reproductive age to do the screening. Purpose: To determine the relationship between family support and the readiness of reproductive age women in doing premarital screening at Public Health Center II Sukawati. Method: This study employed correlational analytical design with a cross-sectional approach. There were 104 women of reproductive age which were selected by using consecutive sampling techniques. The data were collected through questionnaires and analyzed by using Spearman’s Rho test. Findings: The results showed that the majority of respondents had high family support with an average support score was 41.88 and a mode score was 51, as well as good readiness with an average score was 7.79. The statistical analysis showed significant relationship between family support and the readiness of reproductive age women in doing premarital screening (p< 0.001; r = 0.662). Conclusion: There is significant relationship between family support and the readiness of reproductive age women in doing premarital screening at Public Health Center II Sukawati. It is necessary to improve education and encourage active family involvement in supporting premarital screening to create a healthy and high-quality generation. Keywords: Family Support, Readiness, Reproductive Age Woman, Premarrital Screening