Konsumsi alkohol berlebihan dapat meningkatkan kadar kolesterol total yang menyebabkan terjadinya stress oksidatif pemicu kerusakan hati. Prevalensi Alcohol Related Liver Diseases di dunia sebesar 4,8% dengan terapi penggunaan obat statin yang memiliki efek samping berupa myalgia. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak kulit anggur Bali (Vitis vinifera L. Var Alphonso Lavallae) dalam menurunkan kolesterol total dan dosis yang paling optimal, serta pengaruhnya terhadap histologi hepar mencit (Mus musculus L.) yang diinduksi alkohol. Desain penelitian ini yaitu true experimental dengan pretest-postest control group design pada 30 ekor Mus musculus L. dengan kelompok K-, K+ dan tiga dosis ekstrak kulit anggur Bali (100, 350, dan 500 mg/kgBB). Analisis data menggunakan SPSS statistic versi 20, dengan dilakukan uji One Way Anova dan uji Post Hoc Bonferroni. Hasil penelitian menunjukan bahwa ekstrak kulit anggur bali mengandung Flavonoid, Steroid, Alkaloid, Terpenoid dan Fenol. Dari ketiga dosis tersebut diketahui mampu menurunkan kadar kolesterol total dan memperbaiki struktur histologi hepar Mus musculus L. Ekstrak kulit anggur bali yang paling efektif dalam menurunkan kolesterol total dan memperbaiki sel nekrosis hepar mencit yang setara dengan kontrol positif yaitudosis350mg/kgBB. Katakunci:AnggurBali,histologihepar,kolesteroltotal

Consuming excessive alcohol can increase total cholesterol levels and make oxidative stress that triggers liver damage. The prevalence of Alcohol-Related Liver Diseases in the world is 4.8%, with statin therapy commonly used which has its side effects such as myalgia. To determine the activity of Balinese grapes peel extract (Vitis vinifera L. Var. Alphonso Lavallae) in reducing total cholesterol levels and to determine the most optimal dose, as well as its effect on the liver histology of mice Mus musculus L.) which was induced by alcohol. This study employed a true experimental design with a pretest-post test control group design on 30 Mus musculus L., divided into (K−) group, (K+) group, and three groups with Balinese grapes peel extract at doses of 100, 350, and 500 mg/kgBW. The data were analysed by using SPSS Statistics version 20, with One-Way ANOVA followed by a Post Hoc Bonferroni test. The results of the research showed that Balinese grape peel extract contained flavonoids, steroids, alkaloids, terpenoids, and phenols. All three doses were effective in reducing total cholesterol levels and improving the liver histological structure of Mus musculus L. The most effective dose of Balinese grapes peel extract in reducing total cholesterol and improving necroticlivercellsinmicecomparabletothepositivecontrolis350mg/kgBW. Keywords:BalineseGrapes,liverhistology,TotalCholesterol