Latar Belakang: Diabetes mellitus adalah penyakit metabolik dengan karakteristik kadar gula darah tinggi (> 200 mg/dL). Daun Afrika (Vernonia amygdalina Del.) diketahui mengandung berbagai senyawa aktif yang berpotensi sebagai antidiabetes. Tujuan: Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dan hipoglikemi ekstrak etanol daun Afrika (Vernonia amygdalina Del.) terhadap mencit (Mus musculus L.) yang diinduksi aloksan. Metode:2 Penelitian ini merupakan eksperimen laboratorium dengan desain pretest-posttest only group. Model hewan hiperglikemi diperoleh dengan induksi aloksan 150 mg/kgBB. Hewan coba dibagi menjadi 4 kelompok (K+, K-, P1 (dosis 150 mg/kgBB), P2 (dosis 300 mg/kgBB)) dengan metode simple random sampling. Penelitian ini menguji kandungan kimia, aktivitas antioksidan, dan aktivitas penurunan glukosa darah hewan coba. Analisis data menggunakan SPSS versi 29 for windows, diawali dengan uji normalitas (Shapiro-Wilk) dan homogenitas (Levene Test), dilanjutkan dengan One Way ANOVA dan post-hoc Bonferroni. Hasil: Ekstrak daun Afrika positif mengandung flavonoid, alkaloid, tanin, dan saponin. Ekstrak daun Afrika diketahui memiliki aktivitas antioksidan (123,732 μg/mL). Dosis 150 mg/kgBB dan 300 mg/kgBB ekstrak daun Afrika diketahui dapat menurunkan kadar glukosa darah. Kesimpulan: Ekstrak etanol 96% daun Afrika mengandung flavonoid, alkaloid, tanin, dan saponin serta memiliki aktivitas antioksidan sedang. Dosis efektif dalam menurunkan glukosa darah adalah dosis 300 mg/kgBB. Kata Kunci: Aloksan, (Vernonia amydalina Del.), Diabetes mellitus, antioksidan, mencit Jantan (Mus musculus L.)

Background: Diabetes mellitus is a metabolic disease characterized by high blood sugar levels (> 200 mg/dL). African leaves (Vernonia amygdalina Del.) are believed to contain various active compounds that have potential as antidiabetic agents. Purpose: To determine the antioxidant and hypoglycemic activities of African leaf (Vernonia amygdalina Del.) ethanol extract on alloxan-induced diabetic mice (Mus musculus L.). Method: This study employed a laboratory experiment with a pretest-posttest only group design. The hyperglycemic animal model was obtained by inducing alloxan at 150 mg/kg body weight. The experimental animals were divided into four groups (K+, K-, P1 (dose 150 mg/kg body weight), and P2 (dose 300 mg/kg body weight)) using simple random sampling. This study examined the chemical content, antioxidant activity, and blood glucose-lowering activity of the test animals. The data was analyzed using SPSS version 29 for Windows, starting with tests to check for normal distribution and equal variance, and then using one-way ANOVA and post-hoc Bonferroni tests. Result: The findings showed that the extract from African leaves contained flavonoids, alkaloids, tannins, and saponins. African leaf extract demonstrated antioxidant activity at an amount of 123,732 μg/mL. Doses of 150 mg/kg body weight and 300 mg/kg body weight of African leaf extract were observed to decrease blood glucose levels. Conclusion: The 96% ethanol extract of African leaves contains flavonoids, alkaloids, tannins, and saponins, demonstrating moderate antioxidant activity. A dosage of 300 mg/kgBW is effective in decreasing blood glucose. Keywords: Aloksan, (Vernonia amydalina Del.), diabetes mellitus, antioxidant, male mice (Mus musculus L.)