Latar Belakang: Diabetes melitus merupakan penyakit metabolik kronis yang ditandai dengan peningkatan kadar glukosa darah. Penggunaan obat konvensional seperti metformin dapat menimbulkan efek samping, sehingga diperlukan alternatif dari bahan alami. Buah Doum (Hyphaene thebaica) mengandung senyawa flavonoid, alkaloid, tanin, dan terpenoid yang berpotensi sebagai antidiabetes. Tujuan Penelitian: Mengetahui aktivitas antidiabetes ekstrak buah Doum terhadap kadar glukosa darah mencit jantan (Mus musculus) yang diinduksi aloksan. Metode: Penelitian eksperimental dengan rancangan pretest-posttest control group menggunakan 30 ekor mencit jantan yang dibagi menjadi 5 kelompok. Kelompok perlakuan diberi ekstrak buah Doum dosis 200, 400, dan 800 mg/kgBB secara oral selama 21 hari. Kadar glukosa darah diukur pada hari ke0, 7, 14, dan 21, lalu dianalisis menggunakan uji ANOVA dan Post Hoc Bonferroni. Hasil: Ekstrak buah Doum menurunkan kadar glukosa darah secara signifikan (p0,05). Kesimpulan: Ekstrak buah Doum dosis 400 mg/kgBB memiliki aktivitas antidiabetes paling optimal dan berpotensi sebagai terapi alternatif. Kata Kunci: Antidiabetes, Buah Doum, Glukosa Darah.

Background: Diabetes mellitus is a chronic metabolic disease characterized by elevated blood glucose levels. The conventional use of drugs such as metformin may cause side effects, highlighting the need for herbal alternatives. The doum fruit (Hyphaene Thebaica) contains flavonoids, alkaloids, tannins, and terpenoids, which show antidiabetic potential. Purpose: To determine the antidiabetic activity of doum fruit extract on blood glucose levels in male mice (Mus musculus) induced by alloxan. Method: This study employed an experimental study with a pretest-posttest control group design using 30 male mice divided into 5 groups. The treatment groups were administered doum fruit extract at doses of 200, 400, and 800 mg/kg body weight orally for 21 days. Blood glucose levels were measured on days 0, 7, 14, and 21, then analyzed using ANOVA and post hoc Bonferroni tests. Result: The finding showed that the doum fruit extract significantly reduced blood glucose levels (p0.05). Conclusion: The extract of Doum fruit at a dosage of 400 mg/kgBW has the most effective antidiabetic activity and shows potential as an alternative therapeutic alternative. Keywords: antidiabetic, doum fruit, blood glucose.