Latar Belakang: Luka insisi merupakan luka terbuka yang umum terjadi dan memerlukan penanganan efektif untuk mempercepat proses penyembuhan jaringan. Bunga Gemitir (Tagetes erecta L.) mengandung senyawa aktif seperti flavonoid, saponin, tanin, dan karotenoid yang memiliki aktivitas antiinflamasi, antioksidan, serta mampu merangsang angiogenesis dan pembentukan kolagen. Tujuan: Penelitian ini bertujuan mengevaluasi mutu fisik dan efektivitas krim ekstrak Bunga Gemitir dalam mempercepat penyembuhan luka insisi pada tikus jantan galur wistar Metode: Menggunakan desain post-test only control group dengan enam kelompok perlakuan (kontrol normal, kontrol negatif, kontrol positif (povidone iodine) dan krim ekstrak 0,5%, 1%, 2%). Observasi dilakukan pada hari ke-7 dan ke-14 dengan parameter mutu fisik, panjang luka, dan evaluasi histopatologi. Data persentase penyembuhan dianalisis dengan Two Way ANOVA dan post hoc Bonferroni, sedangkan data histopatologi dianalisis dengan Kruskal-Wallis dan Mann-Whitney. Parameter yang dianalisis meliputi mutu fisik sediaan (organoleptis, homogenitas, pH, daya sebar), panjang luka, serta histopatologi jaringan (infiltrasi sel radang, angiogenesis, dan kerapatan kolagen). Hasil: Hasil penelitian menunjukkan bahwa krim ekstrak Bunga Gemitir dengan konsentrasi 0,5%, 1% dan 2% memberikan efek penyembuhan signifikan, termasuk penurunan infiltrasi sel radang, peningkatan pembuluh darah baru, dan peningkatan kepadatan kolagen. Kesimpulan: Berdasarkan hasil penelitian, krim ekstrak Bunga Gemitir efektif dalam mempercepat proses penyembuhan luka insisi dan berpotensi dikembangkan sebagai terapi fitofarmaka. Kata kunci: Tagetes erecta L., Krim, Luka Insisi, Penyembuhan Luka, Hispatologi Jaringan

Background: Incision wounds are a common type of open wound that requires effective treatment to enhance tissue healing. Marigold (Tagetes erecta L.) flowers contain active compounds such as flavonoids, saponins, tannins, and carotenoids, which exhibit anti-inflammatory and antioxidant activities and are capable of stimulating angiogenesis and collagen formation. Purpose: To evaluate the physical quality and effectiveness of marigold flower extract cream in accelerating the healing of incision wounds in male Wistar rats. Method: This study employed A post-test-only control group design included six treatment groups: normal control, negative control, positive control (povidone iodine), and extract creams at concentrations of 0.5%, 1%, and 2%. Observations were conducted on days 7 and 14, focusing on physical quality parameters, wound length, and histopathological evaluation. Wound healing percentages were analyzed using a two-way ANOVA followed by a Bonferroni post hoc test, while histopathological data were analyzed using Kruskal-Wallis and Mann-Whitney tests. The parameters assessed included the physical properties of the cream (such as organoleptic characteristics, homogeneity, pH, and spread ability), wound length, and tissue histopathology (which involved inflammatory cell infiltration, angiogenesis, and collagen density). Result: The results showed that creams made with marigold extract at 0.5%, 1%, and 2% significantly helped wounds heal better by reducing inflammation, increasing new blood vessel growth, and improving collagen density. Conclusion: Marigold flower extract cream is effective in accelerating the healing process of incision wounds and has potential for development as a phytopharmaceutical therapy. Keywords: Tagetes erecta L., Cream, Incision Wound, Wound Healing, Tissue Histopathology