Latar Belakang: Konsumsi makanan tinggi lemak menjadi penyebab utama peningkatan kadar kolesterol total yang menjadi faktor risiko penyakit jantung koroner. Terapi dengan obat kimia dalam jangka panjang dapat menimbulkan efek samping. Tujuan: Penelitian ini bertujuan untuk mengetahui potensi ekstrak kulit anggur Bali (Vitis vinifera L. var. Alphonso Lavallee) dalam menurunkan kolesterol total dan dosis yang paling optimal, serta pengaruhnya terhadap histologi jantung mencit (Mus musculus L.) yang diberi pakan tinggi lemak. Metode: Desain penelitian yang digunakan adalah True Experimental dengan Pretest-Posttest Control Group Design pada 30 ekor Mus musculus L. yang dibagi menjadi lima kelompok, termasuk kontrol normal (KN), kontrol positif (K+), dan tiga dosis ekstrak kulit anggur Bali (100, 350, 500 mg/kgBB). Hasil: Hasil penelitian menunjukkan bahwa ekstrak kulit anggur Bali dengan dosis 500 mg/kgBB memberikan penurunan kolesterol total nilai signifikan pada hari ke21 p=

Background: Consuming high fat food is the main cause of increasing total cholesterol which leads into coronary heart disease. The theraphy with long term chemical drugs may cause some side effects. Purpose: To determine the potential of Balinese grape skin extract (Vitis vinifera L. var. Alphonso Lavallee) in reducing total cholesterol and to determine the most optimal dosage, as well as its effect on heart histology in mice (Mus musculus L.) fed by a high-fat diet. Method: The study employed true experimental design with a pretest-posttest control group design involving 30 Mus musculus L. Subjects which were divided into five groups such as normal control (KN), positive control (K+), and three groups received Balinese grape skin extract at doses of 100, 350, and 500 mg/kgBW). Findings: The results showed that the 500 mg/kgBW dose of Balinese grape skin extract significantly reduced total cholesterol levels on day 21 (p < 0.001). It showed histological structure of the heart within nuclei were in the middle of the cells and the absence of necrosis. Phytochemical screening showed Balinese grape skin extract contained steroids, alkaloids, flavonoids, terpenoids, and phenols. Conclusion: Balinese grape skin extract has the potential to reduce total cholesterol with heart histology has nuclei in the middle of the cells. Keywords: Balinese Grapes, Heart Histology, Total Cholesterol